Introduction of Spheroplasts and L-form bacteria

Spheroplasts:-

           Spheroplasts is the bacteria without peptidoglycan layer in cellwall. This is done by treating gram negative bacteria with lysozyme in a hypertonic medium in the presence of penicillin.

L-Form:-

        L-form bacteria was first of all discovered by Dr Kleinberger in laboratory of Lister Institute in London while studying Streptobacillus moniliformes .The bacteria donot have cell wall. It can be formed from both Gram positive and Gram negative bacteria. This bacteria is formed when the bacteria losses the ability to manufacture peptidoglycan .while studying Streptobacillus it was formed and again reverted to  original bacteria.